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goat anti human sdf 1 ab  (R&D Systems)


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    R&D Systems goat anti human sdf 1 ab
    Goat Anti Human Sdf 1 Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 34 article reviews
    goat anti human sdf 1 ab - by Bioz Stars, 2026-03
    94/100 stars

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    goat anti human sdf 1 ab  (R&D Systems)
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    R&D Systems goat anti human sdf 1 ab
    Goat Anti Human Sdf 1 Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat anti human sdf 1b polyclonal ab  (R&D Systems)
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    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit <t>SDF-1</t> pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, <t>SDF-1b,</t> IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.
    Goat Anti Human Sdf 1b Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit <t>SDF-1</t> pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, <t>SDF-1b,</t> IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.
    Goat Anti Human Anti Sdf 1 Polyclonal Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat anti-human sdf-1 abs  (Santa Cruz Biotechnology)
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    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit <t>SDF-1</t> pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, <t>SDF-1b,</t> IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.
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    FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit SDF-1 pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, SDF-1b, IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Expression of stromal-derived factor-1 is decreased by IL-1 and TNF and in dermal wound healing.

    doi: 10.4049/jimmunol.166.9.5749

    Figure Lengend Snippet: FIGURE 3. Recombinant IL-1a and TNF-a potently inhibit SDF-1 pro- duction by human fibroblasts while increasing the expression of IL-8 and MCP-1. A and B, Primary cultures of human gingival fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of SDF-1 in culture supernatants was measured by ELISA (A) and by a Nalm-6 chemotaxis bioassay (B). p, p , 0.05; pp, p , 0.01 (compared with untreated controls). C–E, Human gingival fibroblasts were stimulated with IL-1a (1 mg/ml) for the indicated period, and levels of mRNA encoding SDF-1a, SDF-1b, IL-8, MCP-1, and GAPDH were determined by RNase protection assay (C). The intensities of the mRNA for GAPDH, SDF-1a, and SDF-1b (D) or IL-8 and MCP-1 (E) were measured, and values for GAPDH were used to normalize for differences in the total amount of RNA analyzed at the indicated time points. F, Primary cultures of human dermal fibroblasts were stimulated with rIL-1a or rTNF-a, and the amount of IL-8 in culture supernatants was measured by ELISA. Data in A, B, and F are presented as the mean of three independent experiments that assayed three different fibroblast donors. Error bars exhibit the SD of the values. Data in C, D, and E are from three independent experiments.

    Article Snippet: Next, 100 ml of a 1 mg/ml solution of biotinylated, goat anti-human SDF-1b polyclonal Ab (R&D Systems) was added.

    Techniques: Recombinant, Expressing, Enzyme-linked Immunosorbent Assay, Chemotaxis Assay, Bioassay, Rnase Protection Assay